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human foreskin fibroblast cell line hff  (ATCC)


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    ATCC human foreskin fibroblast cell line hff
    Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell <t>line</t> <t>HFF-1</t> as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)
    Human Foreskin Fibroblast Cell Line Hff, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1579 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+foreskin+fibroblast+hff+cell+line/HFF-1/pmc13200314-41-0-9
    Average 99 stars, based on 1579 article reviews
    human foreskin fibroblast cell line hff - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Targeting EpCAM expression via near-infrared fluorescent antibodies enables microscopic delineation of primary and recurrent HNSCC"

    Article Title: Targeting EpCAM expression via near-infrared fluorescent antibodies enables microscopic delineation of primary and recurrent HNSCC

    Journal: BMC Cancer

    doi: 10.1186/s12885-026-16172-2

    Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)
    Figure Legend Snippet: Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)

    Techniques Used: Expressing, Flow Cytometry, Immunostaining, Labeling

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    Article Snippet: Accepted Manuscript Silibinin-induced apoptosis of breast cancer cells involves mitochondrial impairment Lingling Si, Weiwei Liu, Toshihiko Hayashi, Yachao Ji, Jianing Fu, Yuheng Nie, Kazunori Mizuno, Shunji Hattori, Satoshi Onodera, Takashi Ikejima PII: S0003-9861(19)30098-0 DOI: https://doi.org/10.1016/j.abb.2019.05.009 Reference: YABBI 8007 To appear in: Archives of Biochemistry and Biophysics Received Date: 9 February 2019 Revised Date: 22 April 2019 Accepted Date: 10 May 2019 Please cite this article as: L. Si, W. Liu, T. Hayashi, Y. Ji, J. Fu, Y. Nie, K. Mizuno, S. Hattori, S. Onodera, T. Ikejima, Silibinin-induced apoptosis of breast cancer cells involves mitochondrial impairment, Archives of Biochemistry and Biophysics (2019), doi: https://doi.org/10.1016/ j.abb.2019.05.009.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Cell Culture:

    Article Title: Silibinin-induced apoptosis of breast cancer cells involves mitochondrial impairment.
    Article Snippet: Accepted Manuscript Silibinin-induced apoptosis of breast cancer cells involves mitochondrial impairment Lingling Si, Weiwei Liu, Toshihiko Hayashi, Yachao Ji, Jianing Fu, Yuheng Nie, Kazunori Mizuno, Shunji Hattori, Satoshi Onodera, Takashi Ikejima PII: S0003-9861(19)30098-0 DOI: https://doi.org/10.1016/j.abb.2019.05.009 Reference: YABBI 8007 To appear in: Archives of Biochemistry and Biophysics Received Date: 9 February 2019 Revised Date: 22 April 2019 Accepted Date: 10 May 2019 Please cite this article as: L. Si, W. Liu, T. Hayashi, Y. Ji, J. Fu, Y. Nie, K. Mizuno, S. Hattori, S. Onodera, T. Ikejima, Silibinin-induced apoptosis of breast cancer cells involves mitochondrial impairment, Archives of Biochemistry and Biophysics (2019), doi: https://doi.org/10.1016/ j.abb.2019.05.009.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    Modification:

    Article Title: Silibinin-induced apoptosis of breast cancer cells involves mitochondrial impairment.
    Article Snippet: Accepted Manuscript Silibinin-induced apoptosis of breast cancer cells involves mitochondrial impairment Lingling Si, Weiwei Liu, Toshihiko Hayashi, Yachao Ji, Jianing Fu, Yuheng Nie, Kazunori Mizuno, Shunji Hattori, Satoshi Onodera, Takashi Ikejima PII: S0003-9861(19)30098-0 DOI: https://doi.org/10.1016/j.abb.2019.05.009 Reference: YABBI 8007 To appear in: Archives of Biochemistry and Biophysics Received Date: 9 February 2019 Revised Date: 22 April 2019 Accepted Date: 10 May 2019 Please cite this article as: L. Si, W. Liu, T. Hayashi, Y. Ji, J. Fu, Y. Nie, K. Mizuno, S. Hattori, S. Onodera, T. Ikejima, Silibinin-induced apoptosis of breast cancer cells involves mitochondrial impairment, Archives of Biochemistry and Biophysics (2019), doi: https://doi.org/10.1016/ j.abb.2019.05.009.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

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    Article Snippet: Human Foreskin Fibroblast (HFF) cell line was obtained from ATCC Cell Lines (Wesel, Germany).

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    Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell <t>line</t> <t>HFF-1</t> as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)
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    Image Search Results


    Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)

    Journal: BMC Cancer

    Article Title: Targeting EpCAM expression via near-infrared fluorescent antibodies enables microscopic delineation of primary and recurrent HNSCC

    doi: 10.1186/s12885-026-16172-2

    Figure Lengend Snippet: Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)

    Article Snippet: Human foreskin fibroblast cell line HFF-1 was purchased from ATCC (Manassas, USA; SCRC-1041).

    Techniques: Expressing, Flow Cytometry, Immunostaining, Labeling

    Cell compatibility of CoPc‐Lig NPs. A) Cell viability of fibroblasts (black bars) and keratinocytes (white bars) exposed to different concentrations of CoPc‐Lig NPs for 24 h. B) Confocal images showing cell internalization of CoPc‐Lig NPs (0.5 mg/mL) by fibroblasts and keratinocytes. The dark areas in brightfield images represent CoPc‐Lig NPs, while the fluorescence channels show cell nuclei (Blue), and cell cytoskeleton (green).

    Journal: Macromolecular Bioscience

    Article Title: Lignin Nanoparticles Containing Cobalt‐Cyanine Complexes: Potential Multifunctional Platforms for Photoacoustic Imaging and Photothermal Treatment of Bacterial Biofilms in Chronic Wounds

    doi: 10.1002/mabi.202500532

    Figure Lengend Snippet: Cell compatibility of CoPc‐Lig NPs. A) Cell viability of fibroblasts (black bars) and keratinocytes (white bars) exposed to different concentrations of CoPc‐Lig NPs for 24 h. B) Confocal images showing cell internalization of CoPc‐Lig NPs (0.5 mg/mL) by fibroblasts and keratinocytes. The dark areas in brightfield images represent CoPc‐Lig NPs, while the fluorescence channels show cell nuclei (Blue), and cell cytoskeleton (green).

    Article Snippet: Bacterial strains ( Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 9027) and human fibroblast cell line (ATCC‐SCRC‐1041, HFF‐1) were purchased from the American Type Culture Collection (ATCC LGC Standards, Italy).

    Techniques: Fluorescence